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Expansion of central memory T cells (T CM ) in HS skin. Flow cytometric analysis of total CD4+ and CD8+ T cell subsets in (A) the skin and (B) peripheral blood. Frequency of naïve and memory subsets within the CD4+ and CD8+ compartments are shown for (C) skin and (D) peripheral blood. Each dot represents an individual donor (Healthy, diamonds; HS, circles); bars indicate the mean frequency of the population relative to the indicated gate ± standard deviation (SD). Healthy <t>PBMCs,</t> n = 8; Healthy skin, n = 5; HS skin and PBMCs matched samples ( n = 5). Detailed gating strategies for T cell memory subsets (Naïve, T CM , T EM and T RM ) are provided in <xref ref-type=Supplementary Figure 1 . * P < 0.05, ** P < 0.01 and *** P < 0.001 (Two-way ANOVA with Šídák’s multiple comparisons test). " width="250" height="auto" />
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Expansion of central memory T cells (T CM ) in HS skin. Flow cytometric analysis of total CD4+ and CD8+ T cell subsets in (A) the skin and (B) peripheral blood. Frequency of naïve and memory subsets within the CD4+ and CD8+ compartments are shown for (C) skin and (D) peripheral blood. Each dot represents an individual donor (Healthy, diamonds; HS, circles); bars indicate the mean frequency of the population relative to the indicated gate ± standard deviation (SD). Healthy <t>PBMCs,</t> n = 8; Healthy skin, n = 5; HS skin and PBMCs matched samples ( n = 5). Detailed gating strategies for T cell memory subsets (Naïve, T CM , T EM and T RM ) are provided in <xref ref-type=Supplementary Figure 1 . * P < 0.05, ** P < 0.01 and *** P < 0.001 (Two-way ANOVA with Šídák’s multiple comparisons test). " width="250" height="auto" />
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Expansion of central memory T cells (T CM ) in HS skin. Flow cytometric analysis of total CD4+ and CD8+ T cell subsets in (A) the skin and (B) peripheral blood. Frequency of naïve and memory subsets within the CD4+ and CD8+ compartments are shown for (C) skin and (D) peripheral blood. Each dot represents an individual donor (Healthy, diamonds; HS, circles); bars indicate the mean frequency of the population relative to the indicated gate ± standard deviation (SD). Healthy <t>PBMCs,</t> n = 8; Healthy skin, n = 5; HS skin and PBMCs matched samples ( n = 5). Detailed gating strategies for T cell memory subsets (Naïve, T CM , T EM and T RM ) are provided in <xref ref-type=Supplementary Figure 1 . * P < 0.05, ** P < 0.01 and *** P < 0.001 (Two-way ANOVA with Šídák’s multiple comparisons test). " width="250" height="auto" />
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Expansion of central memory T cells (T CM ) in HS skin. Flow cytometric analysis of total CD4+ and CD8+ T cell subsets in (A) the skin and (B) peripheral blood. Frequency of naïve and memory subsets within the CD4+ and CD8+ compartments are shown for (C) skin and (D) peripheral blood. Each dot represents an individual donor (Healthy, diamonds; HS, circles); bars indicate the mean frequency of the population relative to the indicated gate ± standard deviation (SD). Healthy <t>PBMCs,</t> n = 8; Healthy skin, n = 5; HS skin and PBMCs matched samples ( n = 5). Detailed gating strategies for T cell memory subsets (Naïve, T CM , T EM and T RM ) are provided in <xref ref-type=Supplementary Figure 1 . * P < 0.05, ** P < 0.01 and *** P < 0.001 (Two-way ANOVA with Šídák’s multiple comparisons test). " width="250" height="auto" />
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Expansion of central memory T cells (T CM ) in HS skin. Flow cytometric analysis of total CD4+ and CD8+ T cell subsets in (A) the skin and (B) peripheral blood. Frequency of naïve and memory subsets within the CD4+ and CD8+ compartments are shown for (C) skin and (D) peripheral blood. Each dot represents an individual donor (Healthy, diamonds; HS, circles); bars indicate the mean frequency of the population relative to the indicated gate ± standard deviation (SD). Healthy <t>PBMCs,</t> n = 8; Healthy skin, n = 5; HS skin and PBMCs matched samples ( n = 5). Detailed gating strategies for T cell memory subsets (Naïve, T CM , T EM and T RM ) are provided in <xref ref-type=Supplementary Figure 1 . * P < 0.05, ** P < 0.01 and *** P < 0.001 (Two-way ANOVA with Šídák’s multiple comparisons test). " width="250" height="auto" />
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Expansion of central memory T cells (T CM ) in HS skin. Flow cytometric analysis of total CD4+ and CD8+ T cell subsets in (A) the skin and (B) peripheral blood. Frequency of naïve and memory subsets within the CD4+ and CD8+ compartments are shown for (C) skin and (D) peripheral blood. Each dot represents an individual donor (Healthy, diamonds; HS, circles); bars indicate the mean frequency of the population relative to the indicated gate ± standard deviation (SD). Healthy <t>PBMCs,</t> n = 8; Healthy skin, n = 5; HS skin and PBMCs matched samples ( n = 5). Detailed gating strategies for T cell memory subsets (Naïve, T CM , T EM and T RM ) are provided in <xref ref-type=Supplementary Figure 1 . * P < 0.05, ** P < 0.01 and *** P < 0.001 (Two-way ANOVA with Šídák’s multiple comparisons test). " width="250" height="auto" />
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Expansion of central memory T cells (T CM ) in HS skin. Flow cytometric analysis of total CD4+ and CD8+ T cell subsets in (A) the skin and (B) peripheral blood. Frequency of naïve and memory subsets within the CD4+ and CD8+ compartments are shown for (C) skin and (D) peripheral blood. Each dot represents an individual donor (Healthy, diamonds; HS, circles); bars indicate the mean frequency of the population relative to the indicated gate ± standard deviation (SD). Healthy <t>PBMCs,</t> n = 8; Healthy skin, n = 5; HS skin and PBMCs matched samples ( n = 5). Detailed gating strategies for T cell memory subsets (Naïve, T CM , T EM and T RM ) are provided in <xref ref-type=Supplementary Figure 1 . * P < 0.05, ** P < 0.01 and *** P < 0.001 (Two-way ANOVA with Šídák’s multiple comparisons test). " width="250" height="auto" />
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Expansion of central memory T cells (T CM ) in HS skin. Flow cytometric analysis of total CD4+ and CD8+ T cell subsets in (A) the skin and (B) peripheral blood. Frequency of naïve and memory subsets within the CD4+ and CD8+ compartments are shown for (C) skin and (D) peripheral blood. Each dot represents an individual donor (Healthy, diamonds; HS, circles); bars indicate the mean frequency of the population relative to the indicated gate ± standard deviation (SD). Healthy <t>PBMCs,</t> n = 8; Healthy skin, n = 5; HS skin and PBMCs matched samples ( n = 5). Detailed gating strategies for T cell memory subsets (Naïve, T CM , T EM and T RM ) are provided in <xref ref-type=Supplementary Figure 1 . * P < 0.05, ** P < 0.01 and *** P < 0.001 (Two-way ANOVA with Šídák’s multiple comparisons test). " width="250" height="auto" />
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Expansion of central memory T cells (T CM ) in HS skin. Flow cytometric analysis of total CD4+ and CD8+ T cell subsets in (A) the skin and (B) peripheral blood. Frequency of naïve and memory subsets within the CD4+ and CD8+ compartments are shown for (C) skin and (D) peripheral blood. Each dot represents an individual donor (Healthy, diamonds; HS, circles); bars indicate the mean frequency of the population relative to the indicated gate ± standard deviation (SD). Healthy <t>PBMCs,</t> n = 8; Healthy skin, n = 5; HS skin and PBMCs matched samples ( n = 5). Detailed gating strategies for T cell memory subsets (Naïve, T CM , T EM and T RM ) are provided in <xref ref-type=Supplementary Figure 1 . * P < 0.05, ** P < 0.01 and *** P < 0.001 (Two-way ANOVA with Šídák’s multiple comparisons test). " width="250" height="auto" />
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Expansion of central memory T cells (T CM ) in HS skin. Flow cytometric analysis of total CD4+ and CD8+ T cell subsets in (A) the skin and (B) peripheral blood. Frequency of naïve and memory subsets within the CD4+ and CD8+ compartments are shown for (C) skin and (D) peripheral blood. Each dot represents an individual donor (Healthy, diamonds; HS, circles); bars indicate the mean frequency of the population relative to the indicated gate ± standard deviation (SD). Healthy PBMCs, n = 8; Healthy skin, n = 5; HS skin and PBMCs matched samples ( n = 5). Detailed gating strategies for T cell memory subsets (Naïve, T CM , T EM and T RM ) are provided in <xref ref-type=Supplementary Figure 1 . * P < 0.05, ** P < 0.01 and *** P < 0.001 (Two-way ANOVA with Šídák’s multiple comparisons test). " width="100%" height="100%">

Journal: Frontiers in Immunology

Article Title: Reorganization and functional divergence of the CD4+ memory T cell compartment in hidradenitis suppurativa

doi: 10.3389/fimmu.2026.1831664

Figure Lengend Snippet: Expansion of central memory T cells (T CM ) in HS skin. Flow cytometric analysis of total CD4+ and CD8+ T cell subsets in (A) the skin and (B) peripheral blood. Frequency of naïve and memory subsets within the CD4+ and CD8+ compartments are shown for (C) skin and (D) peripheral blood. Each dot represents an individual donor (Healthy, diamonds; HS, circles); bars indicate the mean frequency of the population relative to the indicated gate ± standard deviation (SD). Healthy PBMCs, n = 8; Healthy skin, n = 5; HS skin and PBMCs matched samples ( n = 5). Detailed gating strategies for T cell memory subsets (Naïve, T CM , T EM and T RM ) are provided in Supplementary Figure 1 . * P < 0.05, ** P < 0.01 and *** P < 0.001 (Two-way ANOVA with Šídák’s multiple comparisons test).

Article Snippet: Peripheral blood mononuclear cells (PBMCs) from blood were isolated with StraightFrom ® Whole Blood PBMC Isolation Kit, human (130-126-359, Miltenyi Biotec), and resuspended in the same culture medium as skin cells.

Techniques: Standard Deviation

Heightened pro-inflammatory potential of skin vs peripheral blood CD4+ memory T cells. Frequencies of cytokine-expressing memory T cell subsets from matched skin and PBMC samples ( n = 4). Bars represent the mean percentage (± SD) of cytokine-expressing memory T cells vs parent, under unstimulated baseline conditions (“Unstim”, blue bars/circles) and following stimulation with anti-CD3/anti-CD28 coated beads (“aCD3/aCD28”, red bars/triangles). Data are shown for TNF-α-producing CD4+ T EM (A) and T CM (D) cells, IFN-γ-producing CD4+ T EM (B) and T CM (E) , IL-17A-producing CD4+ T EM (C) and T CM (F) . Statistical differences between skin and PBMCs and stimulation condition were evaluated by 2-way repeated measures ANOVA and Fisher’s LSD test (* P < 0.05 and ** P < 0.01).

Journal: Frontiers in Immunology

Article Title: Reorganization and functional divergence of the CD4+ memory T cell compartment in hidradenitis suppurativa

doi: 10.3389/fimmu.2026.1831664

Figure Lengend Snippet: Heightened pro-inflammatory potential of skin vs peripheral blood CD4+ memory T cells. Frequencies of cytokine-expressing memory T cell subsets from matched skin and PBMC samples ( n = 4). Bars represent the mean percentage (± SD) of cytokine-expressing memory T cells vs parent, under unstimulated baseline conditions (“Unstim”, blue bars/circles) and following stimulation with anti-CD3/anti-CD28 coated beads (“aCD3/aCD28”, red bars/triangles). Data are shown for TNF-α-producing CD4+ T EM (A) and T CM (D) cells, IFN-γ-producing CD4+ T EM (B) and T CM (E) , IL-17A-producing CD4+ T EM (C) and T CM (F) . Statistical differences between skin and PBMCs and stimulation condition were evaluated by 2-way repeated measures ANOVA and Fisher’s LSD test (* P < 0.05 and ** P < 0.01).

Article Snippet: Peripheral blood mononuclear cells (PBMCs) from blood were isolated with StraightFrom ® Whole Blood PBMC Isolation Kit, human (130-126-359, Miltenyi Biotec), and resuspended in the same culture medium as skin cells.

Techniques: Expressing